The Role of TMEM45A in Molecular Mechanism of Cell Differentiation Induced by Retinoic Acid
Abstract
Objective: To study the effect of all-trans retinoic acid on the growth cycle of human keratinocyte line HaCaT and the expression of transmembrane protein 45A at different differentiation stages of HaCaT, so as to clarify the regulatory role of TMEM45A in the proliferation and differentiation of epidermal keratinocytes induced by retinoic acid. Methods: HaCaT cells cultured in vitro was used as the research object. After exposed a certain concentration of ATRA, the changes of cell cycle were detected by flow cytometry, and the expression of TMEM45A at mRNA and protein levels were detected by RT-PCR and immunohistochemical method at different stages. Results: Compared with the control group, the rate of G1 phase was significant increased, while S phase were decreased. Moveover, it was related to the function of time. The expression levels of TMEM45A mRNA and protein decreased gradually with the prolongation of ATRA treatment time. Conclusion: ATRA can inhibit the proliferation of keratinocytes and decrease the expression of TMEM45A at both mRNA and protein levels, suggesting that TMEM45A may play an important roles in maintaining the proliferation of human normal skin.
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DOI: http://dx.doi.org/10.18686/jn.v11i4.216
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